[
  {
    "question": "The major difference between cartilage and bone is that cartilage",
    "subject": "high_school_biology",
    "choices": [
      "is a part of the skeletal system",
      "is composed of collagen and salts",
      "lacks blood vessels and nerves",
      "secretes a matrix"
    ],
    "answer": 2
  },
  {
    "question": "Which of the following best explains why lignin is important to the evolutionary success of land plants?",
    "subject": "high_school_biology",
    "choices": [
      "Lignin provides structural support, allowing plants to grow tall.",
      "Lignin stimulates spore formation, leading to gametogenesis.",
      "Lignin supplies the developing embryo with essential nutrients, enabling its growth.",
      "Lignin supplements the absorption of light by chlorophyll, increasing photosynthetic output."
    ],
    "answer": 0
  },
  {
    "question":"A mutation in a bacterial enzyme changed a previously polar amino acid into a nonpolar amino acid. This amino acid was located at a site distant from the enzyme\u2019s active site. How might this mutation alter the enzyme\u2019s substrate specificity?",
    "subject":"high_school_biology",
    "choices":[
      "By changing the enzyme\u2019s pH optimum",
      "By changing the enzyme\u2019s location in the cell",
      "By changing the shape of the protein",
      "An amino acid change away from the active site cannot alter the enzyme\u2019s substrate specificity."
    ],
    "answer":2
  }
]