{
    "protocol": "To synthesize hydrophilic polymers with functional groups, mix 12 precursor polymers functionalized with hydrazide groups and 2 functionalized with aldehyde groups in various ratios. Utilize a high-content robotics system for automated mixing to fabricate 126 different hydrogels within 25 minutes. Ensure the selected precursor polymers exhibit various charges (anionic, cationic, neutral), degradabilities (naturally sourced vs synthetic), and thermoresponsiveness (utilizing PNIPAM and POEGMA with different LCST values).1. Synthesize hydrazide functionalized POEGMA by mixing diethylene glycol methacrylate, oligo ethylene glycol methacrylate, acrylic acid, AIBMe, TGA, and 20 mL of dioxane in a 50 mL Schlenk three-neck flask. Purge with nitrogen for 30 min. Place the flask in a 75 °C oil bath and stir for 4 hours. 2. Remove excess dioxane by rotary evaporation and redissolve poly(OEGMA-co-AA) in 100 mL of distilled deionized water (DIW). 3. Functionalize acrylic acid residues with hydrazide groups using 5-fold excess of adipic acid dihydrazide and 2.5-fold excess of EDC at pH 4.75. 4. Dialyze for purification (6 hours for 6 cycles, MWCO = 3.5 kDa). 5. Characterize polymers using 1H NMR and conductometric titration to determine net cationic and anionic charge content."
}