{
    "protocol": "1. Synthesize POSS-P(QAC-co-AEMA) and P(HEAA-co-GMA) copolymers. 2. Incorporate AEMA and GMA to crosslink the two random copolymers. 3. Add a small quantity of 1,3,5-triformylbenzene (TFB) as a co-crosslinker to react with amino and hydroxyl groups in the copolymers and amino-modified glass substrates. 4. Blend copolymers with varying ratios: For one-component coatings, use POSS-P(QAC-co-AEMA) or P(HEAA-co-GMA) only. For blending coatings, utilize different mass ratios as follows: PPQA (POSS-P(QAC-co-AEMA) 18 mg, TFB 0.54 mg), PPQA/PHG (12 mg POSS-P(QAC-co-AEMA), 6 mg P(HEAA-co-GMA), TFB 0.54 mg), PPQA/PHG (9 mg POSS-P(QAC-co-AEMA), 9 mg P(HEAA-co-GMA), TFB 0.54 mg), PPQA/PHG (6 mg POSS-P(QAC-co-AEMA), 12 mg P(HEAA-co-GMA), TFB 0.54 mg), PHG (18 mg P(HEAA-co-GMA), TFB 0.54 mg). 5. Evaluate antifogging and antibacterial properties using hot-vapor and cold-warm antifogging tests, standard plate count method, bacterial antiadhesive assays, and growth inhibition.Synthesis of POSS-P(QAC-co-AEMA) and P(HEAA-co-GMA) Copolymers was carried out using reversible addition−fragmentation chain transfer (RAFT) polymerization for POSS-P(QAC-co-AEMA) and free radical polymerization for P(HEAA-co-GMA). QAC was prepared by quaternization of 2-(dimethylamino)ethyl methacrylate (DMAEMA) with 1-bromobutane. The hydrophobic component was incorporated by modifying the RAFT agent of CPADB with aminopropylisobutyl polyhedral oligomeric silsesquioxane (POSS-NH2) to obtain POSS-CPADB. For blending coatings, bare glass was treated with oxygen plasma (18 W, 60 s) and immersed in (3-aminopropyl)-trimethoxysilane/methanol solution (5%, v/v) for 4 h. After sonicating with methanol and ethanol, copolymers with varying POSS-P(QAC-co-AEMA)/P(HEAA-co-GMA) blending ratios were dissolved in 200 µL of ethanol. A determined volume of the mixture was drop-coated onto the modified glass slides to allow crosslinking for antifogging tests. Coated samples were dried at room temperature and thermal cured at 40 °C overnight.Synthesis procedures of QAC, POSS-CPADB, POSS-P(QAC-co-AEMA), and P(HEAA-co-GMA) with functional groups are detailed in the Supporting Information, including their characterizations by $^{1}\\mathrm{H}$ NMR and GPC."
}