{
    "protocol": "Prepare a semi-interpenetrating polymer network (SIPN) using a linear terpolymer poly(2-(dimethylamino)ethyl methacrylate-co-N-vinylpyrrolidone-co-methyl methacrylate) at an optimal molar ratio of 40/30/30 for DMAEMA/NVP/MMA, combined with a network of poly(ethylene glycol dimethacrylate) (PEGDMA).Monomers including DMAEMA (98%), N-vinylpyrrolidone (NVP, 99%), EGDMA (99%), and MMA (99%) are dissolved in a suitable solvent such as DMF, toluene, or chloroform. Free radical initiators, HHMP (98%) or AIBN (99%), are added to the solution to initiate the polymerization. The reaction mixture is stirred at an appropriate temperature and for a specific duration to allow the formation of the hydrophilic polymers with desired functional groups.Three monomers, DMAEMA (4.8 g), NVP (4.5 g) and MMA (3.0 g) (molar percentage: 30% DMAEMA, 40% NVP, and 30% MMA) were added into DMF in a 100 mL flask to obtain 10 wt% solution; then, the thermal initiator AIBN (0.5 wt% with respect to the total monomer mass) was added. After being purged by argon for 20 min, the polymerization was carried out at 70°C for 24 h under magnetic stirring (200 rpm). The final terpolymer, designated as T-30 according to the DMAEMA molar percentage, was purified by dissolution in chloroform and precipitation in cyclohexane (twice), and dried in a vacuum oven.Glass slides (1.5 × 1.5 cm²) were sonicated consecutively in acetone and ethanol for 30 min, followed by blow-drying with air. A terpolymer (2.0 g), EGDMA (0.5 wt% with respect to the terpolymer), and HHMP (2.0 wt% relative to EGDMA) were dissolved in toluene (20.0 mL) to obtain a homogeneous solution. The mixture was then spun-coated on clean glass slides at various rates (400, 800, 1500 and 3000 rpm) for 15 s. The spun-coated film was then cured under UV irradiation in a UVP CL-1000 ultraviolet cross-linker apparatus (365 nm, 15 W) for 45 min, and dried in a vacuum oven at 70 °C for 24 h.Synthesize terpolymers using three monomers: DMAEMA, NVP, and MMA through conventional free radical polymerization. The molar ratio of the monomers should be adjusted to achieve a balanced hydrophobicity and hydrophilicity in the final terpolymer. After synthesis, form a SIPN coating comprising the terpolymer and a network of polymerized EGDMA at a concentration of 0.5 wt% relative to the copolymer. Monitor the molecular weight of the terpolymers using GPC, aiming for an Mn of approximately 30,000, and confirm the molar ratio of the monomeric units using 1H-NMR. Adjust the Tg of the terpolymer by varying the composition, particularly increasing the NVP content while keeping MMA fixed at about 30 mol%."
}